ER-GCaMP6f-AAV
(Plasmid
#182501)
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PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV5
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Vector typeAAV
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameER localized P450
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Insert Size (bp)81
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ER-GCaMP6f-AAV was a gift from Chris Richards (Addgene plasmid # 182501 ; http://n2t.net/addgene:182501 ; RRID:Addgene_182501) -
For your References section:
ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes. Aryal SP, Xia M, Adindu E, Davis C, Ortinski PI, Richards CI. Anal Chem. 2022 Jan 21. doi: 10.1021/acs.analchem.1c04321. 10.1021/acs.analchem.1c04321 PubMed 35061939