pCDNA_MET_D14_3Flag
(Plasmid
#182495)
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PurposeContains cDNA of the MET gene without the exon 14
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1+
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMET
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SpeciesH. sapiens (human)
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Insert Size (bp)4029
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MutationExon 14 deleted
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Entrez GeneMET (a.k.a. AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met)
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Tag
/ Fusion Protein
- C-terminal 3xFlag tag (C terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA_MET_D14_3Flag was a gift from Paolo Comoglio (Addgene plasmid # 182495 ; http://n2t.net/addgene:182495 ; RRID:Addgene_182495) -
For your References section:
MET14 promotes a ligand-dependent, AKT-driven invasive growth. Cerqua M, Botti O, Arigoni M, Gioelli N, Serini G, Calogero R, Boccaccio C, Comoglio PM, Altintas DM. Life Sci Alliance. 2022 May 30;5(10). pii: 5/10/e202201409. doi: 10.26508/lsa.202201409. Print 2022 Oct. 10.26508/lsa.202201409 PubMed 35636967
Map uploaded by the depositor.
