Coexpressed Free PcPTS
(Plasmid #182483)

• Purpose: Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
• Depositing Lab: Claudia Vickers
• Publication: Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.1016/j.ymben.2023.03.012. Epub 2023 Mar 28.

Backbone

• Vector backbone: pILGFPB5A
• Vector type: Yeast Expression, Synthetic Biology ; Metabolic engineering
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: FPPS
• Alt name: ERG20, wtERG20, farnesyl diphosphate synthase
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1059
• Promoter: GAL10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGTAATGCCATGTAATATGATTATTAAAC
• 3′ sequencing primer: CTCAACAGTGCTCCGAAG

Gene/Insert 2

• Gene/Insert name: PTS
• Alt name: PcPTS, patchoulol synthase
• Species: Synthetic; Pogostemon cablin
• Insert Size (bp): 1659
• Promoter: GAL7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAGTATTCGTTTGGTAAAGTAGAGG
• 3′ sequencing primer: GCATTGGCACGGTGCAACAC

Resource Information

• Supplemental Documents:
  • ERG20 + PcPTS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.11.08.515726v1 for bioRxiv preprint.