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Addgene

Coexpressed Free NES
(Plasmid #182482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILGFPB5A
  • Vector type
    Yeast Expression, Synthetic Biology ; Metabolic engineering
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FPPS
  • Alt name
    ERG20, wtERG20, farnesyl diphosphate synthase
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    1059
  • Promoter GAL10

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGTAATGCCATGTAATATGATTATTAAAC
  • 3′ sequencing primer CTCAACAGTGCTCCGAAG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    NES
  • Alt name
    AcNES1, nerolidol/linalool synthase
  • Species
    Synthetic; Actinidia chinensis
  • Insert Size (bp)
    1721
  • Promoter GAL7

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAGTATTCGTTTGGTAAAGTAGAGG
  • 3′ sequencing primer GCATTGGCACGGTGCAACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Coexpressed Free NES was a gift from Claudia Vickers (Addgene plasmid # 182482 ; http://n2t.net/addgene:182482 ; RRID:Addgene_182482)

  • For your References section:

    Metabolic flux enhancement from the translational fusion of terpene synthases is linked to terpene synthase accumulation. Cheah LC, Liu L, Stark T, Plan MR, Peng B, Lu Z, Schenk G, Sainsbury F, Vickers CE. Metab Eng. 2023 May;77:143-151. doi: 10.1016/j.ymben.2023.03.012. Epub 2023 Mar 28. 10.1016/j.ymben.2023.03.012 PubMed 36990382
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