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deltaVP1d63C + VP2C-GFPdeg
(Plasmid #182481)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILGFPB5A
  • Vector type
    Yeast Expression, Synthetic Biology ; Metabolic engineering
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VP2C-GFPdeg
  • Alt name
    VP2C-yEGFPdeg, VP2C-GFPmodc, destabilised GFP, degron-tagged GFP
  • Species
    M. musculus (mouse), Synthetic; Murine polyomavirus
  • Insert Size (bp)
    1038
  • Promoter GAL10

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGTAATGCCATGTAATATGATTATTAAAC
  • 3′ sequencing primer GCATTGGCACGGTGCAACAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    deltaVP1d63C
  • Alt name
    VP1, deltaNLS VP1, C-terminal truncated VP1
  • Species
    Synthetic; Murine polyomavirus
  • Insert Size (bp)
    954
  • Promoter GAL1

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTTCTTATTCAAATGTCATAAAAGTATCAAC
  • 3′ sequencing primer CCAGCCTGCTTTTCTGTAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VP1 has 63 residues removed from the C-terminal. This abolishes in-vivo assembly in yeast.

Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    deltaVP1d63C + VP2C-GFPdeg was a gift from Claudia Vickers (Addgene plasmid # 182481 ; http://n2t.net/addgene:182481 ; RRID:Addgene_182481)

  • For your References section:

    Synthetic in vivo compartmentalisation improves metabolic flux and modulates the product profile of promiscuous enzymes. Cheah LC, Liu L, Plan MR, Peng B, Lu Z, Schenk G, Vickers CE, Sainsbury F. bioRxiv 2022.11.24.517869 10.1101/2022.11.24.517869
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