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Addgene

Linked Free NES (M)
(Plasmid #182436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILGFPB5A
  • Vector type
    Yeast Expression, Synthetic Biology ; Metabolic engineering
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FPPS(M)-NES
  • Alt name
    ERG20(F96W-N127W)-AcNES1, nerolidol/linalool synthase
  • Species
    S. cerevisiae (budding yeast), Synthetic; Actinidia chinensis
  • Insert Size (bp)
    2817
  • Promoter GAL10

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGTAATGCCATGTAATATGATTATTAAAC
  • 3′ sequencing primer GCATTGGCACGGTGCAACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Linked Free NES (M) was a gift from Claudia Vickers (Addgene plasmid # 182436 ; http://n2t.net/addgene:182436 ; RRID:Addgene_182436)

  • For your References section:

    Synthetic in vivo compartmentalisation improves metabolic flux and modulates the product profile of promiscuous enzymes. Cheah LC, Liu L, Plan MR, Peng B, Lu Z, Schenk G, Vickers CE, Sainsbury F. bioRxiv 2022.11.24.517869 10.1101/2022.11.24.517869
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