Coexpressed VLP NES (M)
(Plasmid #182435)

• Purpose: YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
• Depositing Lab: Claudia Vickers
• Publication: Li Chen Cheah et al BioRxiv, 2022

Backbone

• Vector backbone: pILGFPB5A
• Vector type: Yeast Expression, Synthetic Biology ; Metabolic engineering
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VP2C-FPPS(M)
• Alt name: VP2C-ERG20(F96W-N127W), farnesyl diphosphate synthase
• Species: S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
• Insert Size (bp): 1263
• Promoter: GAL10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGTAATGCCATGTAATATGATTATTAAAC
• 3′ sequencing primer: CTCAACAGTGCTCCGAAG

Gene/Insert 2

• Gene/Insert name: deltaVP1
• Alt name: VP1, deltaNLS VP1
• Species: Synthetic; Murine polyomavirus
• Insert Size (bp): 1143
• Promoter: GAL1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACTTCTTATTCAAATGTCATAAAAGTATCAAC
• 3′ sequencing primer: CCAGCCTGCTTTTCTGTAAC

Gene/Insert 3

• Gene/Insert name: VP2C-NES
• Alt name: VP2C-AcNES1, nerolidol/linalool synthase
• Species: Synthetic; Murine polyomavirus; Actinidia chinensis
• Insert Size (bp): 1926
• Promoter: GAL7

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAGTATTCGTTTGGTAAAGTAGAGG
• 3′ sequencing primer: GCATTGGCACGGTGCAACAC

Resource Information

• Supplemental Documents:
  • Coexpressed VLP NES (M).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.