pET41c-PhTET2
(Plasmid
#182428)
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PurposeExpresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET41c
- Backbone size w/o insert (bp) 5186
- Total vector size (bp) 6248
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTetrahedral aminopeptidase TET2 from P. hirokoshii
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Alt nameUniProtKB - O59196
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SpeciesPyrococcus horikoshii
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Insert Size (bp)1062
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe plasmid originates from Bruno Franzetti, Institut de Biologie Structurale Grenoble.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.06.29.450317v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET41c-PhTET2 was a gift from Bruno Franzetti (Addgene plasmid # 182428 ; http://n2t.net/addgene:182428 ; RRID:Addgene_182428) -
For your References section:
Functional control of a 0.5 MDa TET aminopeptidase by a flexible loop revealed by MAS NMR. Gauto DF, Macek P, Malinverni D, Fraga H, Paloni M, Sucec I, Hessel A, Bustamante JP, Barducci A, Schanda P. Nat Commun. 2022 Apr 8;13(1):1927. doi: 10.1038/s41467-022-29423-0. 10.1038/s41467-022-29423-0 PubMed 35395851