pJC20_cam1-T6C
(Plasmid
#182427)
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PurposeBacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182427 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC20
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecam1
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SpeciesS. pombe (fission yeast)
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MutationT6C substitution
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GenBank IDUniProtKB: P05933
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Entrez Genecam1
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC20_cam1-T6C was a gift from Dan Mulvihill (Addgene plasmid # 182427 ; http://n2t.net/addgene:182427 ; RRID:Addgene_182427) -
For your References section:
Acetylation stabilises calmodulin-regulated calcium signalling. Baker K, Geeves MA, Mulvihill DP. FEBS Lett. 2022 Jan 31. doi: 10.1002/1873-3468.14304. 10.1002/1873-3468.14304 PubMed 35100446