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pRSFDuet-1_mNeongreen-Etanercept
(Plasmid #182420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDUET-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeongreen-Etanercept
  • Species
    H. sapiens (human)
  • GenBank ID
    UniProtKB: P20333
  • Entrez Gene
    TNFRSF1B (a.k.a. CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR)
  • Promoter T7
  • Tag / Fusion Protein
    • mNeongreen (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-1_mNeongreen-Etanercept was a gift from Dan Mulvihill (Addgene plasmid # 182420 ; http://n2t.net/addgene:182420 ; RRID:Addgene_182420)

  • For your References section:

    High-yield vesicle-packaged recombinant protein production from E. coli. Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, Brown IR, Hiscock JR, Lennon C, Mulvihill DP. Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27. 10.1016/j.crmeth.2023.100396 PubMed 36936078
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