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Addgene

MMTV-SV40-Bssk
(Plasmid #1824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BlueScribe SK+
  • Backbone size (bp) 7023
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SalI to HindIII MMTV-LTR from PA9; SV40 PA/intron is from CDM8. Unique cloning sites for expression from the LTR are EcoRI and HindIII.
(Leder #C-197)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MMTV-SV40-Bssk was a gift from Philip Leder (Addgene plasmid # 1824 ; http://n2t.net/addgene:1824 ; RRID:Addgene_1824)

  • For your References section:

    Binary system for regulating transgene expression in mice: targeting int-2 gene expression with yeast GAL4/UAS control elements. Ornitz DM, Moreadith RW, Leder P. Proc Natl Acad Sci U S A 1991 Feb 1;88(3):698-702. 10.1073/pnas.88.3.698 PubMed 1846961
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