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pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)
(Plasmid #182368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182368 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDendra2
  • Backbone size w/o insert (bp) 3937
  • Total vector size (bp) 4895
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glyceraldehyde-3-phosphate dehydrogenase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    958
  • GenBank ID
    NM_001289726.1
  • Entrez Gene
    Gapdh (a.k.a. Gapd)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • Pal-Dendra2 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH) was a gift from Chunghun Lim (Addgene plasmid # 182368 ; http://n2t.net/addgene:182368 ; RRID:Addgene_182368)

  • For your References section:

    mtIF3 is locally translated in axons and regulates mitochondrial translation for axonal growth. Lee S, Park D, Lim C, Kim JI, Min KT. BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w. 10.1186/s12915-021-01215-w PubMed 34996455
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