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pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2
(Plasmid #182357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-Amp-high-copy
  • Backbone manufacturer
    Twist Biosciences
  • Total vector size (bp) 5028
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    C. elegans (nematode)
  • Promoter Peft-3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Positive control vector: Peft-3::gfp(NLS)::tbb-2 UTR
Will give unc-119 rescue and ubiquitous GFP expression after insertion into any unc-119 mosTI sites.

Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 182357 ; http://n2t.net/addgene:182357 ; RRID:Addgene_182357)

  • For your References section:

    Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171
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