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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
(Plasmid #182356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist-Amp-high-copy
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Cloning vector for single-copy insertion using unc-119 selection.
MCS/Golden Gate (BsaI) cloning vector with insertion between upstream of gpd-2 operon and mScarlet(NLS)::tbb-2 3' UTR.
Use to monitor expression of transgene by co-expression of fluorophore.
BsaI overhangs: tgcc - mcs - tgag (use tga as the stop site in the BsaI overlap for transgenes).

Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 182356 ; http://n2t.net/addgene:182356 ; RRID:Addgene_182356)

  • For your References section:

    Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171
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