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Addgene

FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
(Plasmid #182346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Hyung-song Nam, Capecchi laboratory
  • Vector type
    Mammalian Expression, Mouse Targeting ; FLP / FRT
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    FNF is from Addgene plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. SV40 pA is from Clontech. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Release the insert with PacI.

Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector was a gift from Mario Capecchi (Addgene plasmid # 182346 ; http://n2t.net/addgene:182346 ; RRID:Addgene_182346)
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