mT2A-TagRFP-T-linker-dre-PBD*-FNF
(Plasmid
#182342)
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PurposeRecombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Backbone manufacturerHyung-song Nam, Capecchi laboratory
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Vector typeMammalian Expression, Mouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2A-TagRFP-T-linker-dre-PBD*-FNF
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTagRFP-T is from Roger Tsien. dre-PBD* is from Francis Stewart. FNF is from Addgene plasmid #22687.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Release the insert with PmeI and PacI digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mT2A-TagRFP-T-linker-dre-PBD*-FNF was a gift from Mario Capecchi (Addgene plasmid # 182342 ; http://n2t.net/addgene:182342 ; RRID:Addgene_182342)