pcDNA3-H2B-μDpTT
(Plasmid #182328)

• Purpose: Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
• Depositing Lab: Jin Zhang
• Publication: Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 7191
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-μDronpa-TagRFP-T
• Alt name: H2B-Dronpa(H193T)-DEVD-TagRFP-T
• Species: H. sapiens (human)
• Insert Size (bp): 1776
• Mutation: Dronpa contains H193T mutation.
• Entrez Gene: H2BC3 (a.k.a. H2B.1, H2B/f, H2BFF, HIST1H2BB)
• Promoter: CMV
• Tag / Fusion Protein:
  • Full-length human histone H2B (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-H2B-Dp(H193T)-TT.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry