pB006
(Plasmid
#182320)
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Purpose(Empty Backbone) It's an empty replication plasmid backbone in Bacteroides
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepB006
- Backbone size (bp) 5025
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Vector typeBacterial Expression, Synthetic Biology
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Selectable markersErythromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E.coli S17-1
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB006 was a gift from Lei Dai (Addgene plasmid # 182320 ; http://n2t.net/addgene:182320 ; RRID:Addgene_182320) -
For your References section:
CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species. Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W, Dai L. ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 10.1021/acssynbio.1c00543. Epub 2022 Jan 6. 10.1021/acssynbio.1c00543 PubMed 34990118