PB-TO-hNIL BSD-mApple
(Plasmid #182313)

• Purpose: Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple
• Depositing Labs: iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
• Publication: Characterization of iPSC lines for the iPSC Neurodegenerative Disease Initiative (iNDI) (unpublished)

Backbone

• Vector backbone: pUCM
• Backbone size w/o insert (bp): 12000
• Total vector size (bp): 16348
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hNGN2
• Species: H. sapiens (human)
• Insert Size (bp): 819
• Entrez Gene: NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)

Gene/Insert 2

• Gene/Insert name: hISL1
• Species: H. sapiens (human)
• Insert Size (bp): 1050
• Entrez Gene: ISL1 (a.k.a. ISLET1, Isl-1)

Gene/Insert 3

• Gene/Insert name: hLHX3
• Species: H. sapiens (human)
• Insert Size (bp): 1209
• Entrez Gene: LHX3 (a.k.a. CPHD3, LIM3, M2-LHX3)

Gene/Insert 4

• Gene/Insert name: bsd-T2A-mycNLS-mApple
• Species: H. sapiens (human)
• Insert Size (bp): 1473
• Tag / Fusion Protein:
  • T2A-mycNLS-mApple

Resource Information

• Supplemental Documents:
  • L72 PB-TO-hNIL BSD-mApple.gbk
• A portion of this plasmid was derived from a plasmid made by: Michael Ward lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry