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Addgene

pMMTV neu NT
(Plasmid #1823)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1823 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neu NT
  • Alt name
    c-neu oncogene
  • Alt name
    MMTV promoter
  • Alt name
    neu
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Erbb2 (a.k.a. HER2/neu, neu)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map.
(Leder #C-172)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMTV neu NT was a gift from Philip Leder (Addgene plasmid # 1823 ; http://n2t.net/addgene:1823 ; RRID:Addgene_1823)

  • For your References section:

    Single-step induction of mammary adenocarcinoma in transgenic mice bearing the activated c-neu oncogene. Muller WJ, Sinn E, Pattengale PK, Wallace R, Leder P. Cell 1988 Jul 1;54(1):105-15. 10.1016/0092-8674(88)90184-5 PubMed 2898299
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