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pQE31-6xHis-Countdown
(Plasmid #182299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE31
  • Backbone size w/o insert (bp) 3435
  • Total vector size (bp) 4152
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Countdown
  • Species
    Echinophyllia sp. SC22
  • Insert Size (bp)
    717
  • Promoter T5
  • Tag / Fusion Protein
    • histidine tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer GAGGCCCTTTCGTCTTCAC
  • 3′ sequencing primer GCCATTGGGATATATCAACGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Read the preprint here: https://andrewgyork.github.io/relaxation_sensors/, doi:10.5281/zenodo.5810930

Please note: Plasmid contains a frameshift and early termination in the CmR (Chloramphenicol) resistance marker likely compromising it's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE31-6xHis-Countdown was a gift from Andrew York (Addgene plasmid # 182299 ; http://n2t.net/addgene:182299 ; RRID:Addgene_182299)

  • For your References section:

    Relaxation Sensors. Seidel ZP, Wang JCK, Riegler J, York AG, Ingaramo M. (2021), doi: 10.5281/zenodo.5810930 10.5281/zenodo.5810930
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