pCDNA3_cAMPFIRE-L-R279E
(Plasmid
#182282)
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PurposeMammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 9846
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3_cAMPFIRE-L-R279E was a gift from Haining Zhong (Addgene plasmid # 182282 ; http://n2t.net/addgene:182282 ; RRID:Addgene_182282) -
For your References section:
Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Massengill CI, Bayless-Edwards L, Ceballos CC, Cebul ER, Cahill J, Bharadwaj A, Wilson E, Qin M, Whorton MR, Baconguis I, Ye B, Mao T, Zhong H. Nat Methods. 2022 Nov;19(11):1461-1471. doi: 10.1038/s41592-022-01646-5. Epub 2022 Oct 27. 10.1038/s41592-022-01646-5 PubMed 36303019
