pSMNot_sra-6::trp-4
(Plasmid
#182261)
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Purposetrp-4 cDNA cloned into pSMNot_sra-6 at Kpn&Notl
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMNot
- Backbone size w/o insert (bp) 3433
- Total vector size (bp) 12868
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nametrp-4
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SpeciesC. elegans (nematode)
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Insert Size (bp)5634
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GenBank IDNM_061028.3
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Entrez Genetrp-4 (a.k.a. CELE_Y71A12B.4)
Gene/Insert 2
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Gene/Insert namesra-6
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SpeciesC. elegans (nematode)
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Insert Size (bp)3801
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GenBank IDLK927569.1
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Entrez Genesra-6 (a.k.a. CELE_AH6.10)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMNot_sra-6::trp-4 was a gift from Sreekanth Chalasani (Addgene plasmid # 182261 ; http://n2t.net/addgene:182261 ; RRID:Addgene_182261) -
For your References section:
Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans. Ibsen S, Tong A, Schutt C, Esener S, Chalasani SH. Nat Commun. 2015 Sep 15;6:8264. doi: 10.1038/ncomms9264. 10.1038/ncomms9264 PubMed 26372413