pUltra-chPheRS
(Plasmid
#182257)
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PurposeGenetic Incorporation of DOPA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182257 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUltra
- Backbone size w/o insert (bp) 4067
- Total vector size (bp) 5882
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namechPheRS2 for DOPA
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Insert Size (bp)1656
Gene/Insert 2
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Gene/Insert namechPheT for DOPA
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Insert Size (bp)72
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUltra-chPheRS was a gift from Han Xiao (Addgene plasmid # 182257 ; http://n2t.net/addgene:182257 ; RRID:Addgene_182257) -
For your References section:
Biosynthesis and Genetic Incorporation of 3,4-Dihydroxy-L-Phenylalanine into Proteins in Escherichia coli. Chen Y, Loredo A, Chung A, Zhang M, Liu R, Xiao H. J Mol Biol. 2021 Dec 20:167412. doi: 10.1016/j.jmb.2021.167412. 10.1016/j.jmb.2021.167412 PubMed 34942167