pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x
(Plasmid
#182256)
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PurposeHuman PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmirGLO Dual-Luciferase
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 7344
- Total vector size (bp) 7946
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150
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SpeciesH. sapiens (human)
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Insert Size (bp)604
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer AGA AGC TGC GCG GTG GTG TTG TG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is a L95R mutation in hRluc. Depositor confirms this does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x was a gift from Silvia Monticelli (Addgene plasmid # 182256 ; http://n2t.net/addgene:182256 ; RRID:Addgene_182256) -
For your References section:
RFX transcription factors control a miR-150/PDAP1 axis that restrains the proliferation of human T cells. Chirichella M, Bianchi N, Dzafo E, Foli E, Gualdrini F, Kenyon A, Natoli G, Monticelli S. PLoS Biol. 2022 Feb 10;20(2):e3001538. doi: 10.1371/journal.pbio.3001538. 10.1371/journal.pbio.3001538 PubMed 35143476