pcDNA ZIP-GFP11
(Plasmid
#182242)
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PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP11
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1
- Total vector size (bp) 5265
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZIP-GFP11
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Alt nameGCN4 peptide
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- split-GFP11 tripartite (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer CMV-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA ZIP-GFP11 was a gift from Stéphanie Cabantous (Addgene plasmid # 182242 ; http://n2t.net/addgene:182242 ; RRID:Addgene_182242) -
For your References section:
A new protein-protein interaction sensor based on tripartite split-GFP association. Cabantous S, Nguyen HB, Pedelacq JD, Koraichi F, Chaudhary A, Ganguly K, Lockard MA, Favre G, Terwilliger TC, Waldo GS. Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038/srep02854. 10.1038/srep02854 PubMed 24092409