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Addgene

pcDNA GFP10-ZIP
(Plasmid #182241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1
  • Total vector size (bp) 5263
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP10-ZIP
  • Alt name
    GCN4 peptide
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • split-GFP10 tripartite (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer CMV-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA GFP10-ZIP was a gift from Stéphanie Cabantous (Addgene plasmid # 182241 ; http://n2t.net/addgene:182241 ; RRID:Addgene_182241)

  • For your References section:

    A new protein-protein interaction sensor based on tripartite split-GFP association. Cabantous S, Nguyen HB, Pedelacq JD, Koraichi F, Chaudhary A, Ganguly K, Lockard MA, Favre G, Terwilliger TC, Waldo GS. Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038/srep02854. 10.1038/srep02854 PubMed 24092409
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