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PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET 28a
- Backbone size w/o insert (bp) 5305
- Total vector size (bp) 5887
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP1-9
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SpeciesSynthetic
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Insert Size (bp)582
- Promoter T7
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Tag
/ Fusion Protein
- 6HIS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 fw
- 3′ sequencing primer T7 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET N6his GFP1-9 was a gift from Stéphanie Cabantous (Addgene plasmid # 182240 ; http://n2t.net/addgene:182240 ; RRID:Addgene_182240) -
For your References section:
High-Throughput Protein-Protein Interaction Assays Using Tripartite Split-GFP Complementation. Pedelacq JD, Waldo GS, Cabantous S. Methods Mol Biol. 2019;2025:423-437. doi: 10.1007/978-1-4939-9624-7_20. 10.1007/978-1-4939-9624-7_20 PubMed 31267465
Map uploaded by the depositor.
