pcDNA GFP10-RHOB
(Plasmid #182237)

• Purpose: Expresses RHOB GTPase with tripartite split GFP10 tag
• Depositing Lab: Stéphanie Cabantous
• Publication: Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 10.1242/jcs.210419.

Backbone

• Vector backbone: pcDNA 3.1
• Backbone size w/o insert (bp): 5119
• Total vector size (bp): 5704
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ras homolog family member B
• Species: H. sapiens (human)
• Insert Size (bp): 588
• GenBank ID: NM_004040.4
• Entrez Gene: RHOB (a.k.a. ARH6, ARHB, MST081, MSTP081, RHOH6)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP10 (split-GFP tripartite) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pcDNA 3.1 rev

Resource Information

• Supplemental Documents:
  • pcdna3 GFP10-RhoB.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry