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Addgene

Akaluc VLP
(Plasmid #182212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pILGFPB5A
  • Vector type
    Yeast Expression, Synthetic Biology ; TEM imaging
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VP2C-Akaluc
  • Species
    S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
  • Insert Size (bp)
    1851
  • Promoter GAL10

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGGTAATGCCATGTAATATGATTATTAAAC
  • 3′ sequencing primer GCATTGGCACGGTGCAACAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
  • Alt name
    MPyV deltaVP1
  • Species
    S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
  • Insert Size (bp)
    1143
  • Promoter GAL1

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTTCTTATTCAAATGTCATAAAAGTATCAAC
  • 3′ sequencing primer CCAGCCTGCTTTTCTGTAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Akaluc VLP was a gift from Claudia Vickers (Addgene plasmid # 182212 ; http://n2t.net/addgene:182212 ; RRID:Addgene_182212)
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