APEX2 VLP
(Plasmid #182211)

• Purpose: Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
• Depositing Lab: Claudia Vickers
• Publication: Plasmids for expressing MPyV VLPs with reporter protein cargo (unpublished)

Backbone

• Vector backbone: pILGFPB5A
• Vector type: Yeast Expression, Synthetic Biology ; TEM imaging
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VP2C-APEX2
• Species: S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
• Insert Size (bp): 951
• Promoter: GAL10

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTGGTAATGCCATGTAATATGATTATTAAAC
• 3′ sequencing primer: GCATTGGCACGGTGCAACAC

Gene/Insert 2

• Gene/Insert name: Murine polyomavirus deltaVP1 (NLS deletion mutant)
• Alt name: MPyV deltaVP1
• Species: S. cerevisiae (budding yeast), Synthetic; Murine polyomavirus
• Insert Size (bp): 1143
• Promoter: GAL1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACTTCTTATTCAAATGTCATAAAAGTATCAAC
• 3′ sequencing primer: CCAGCCTGCTTTTCTGTAAC

Resource Information

• Supplemental Documents:
  • APEX2 VLP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry