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Addgene

pRSET-B-frSkyran-S
(Plasmid #182209)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET-B
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    use BL21(DE3) for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frSkylan-S
  • Species
    Synthetic
  • Insert Size (bp)
    684
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer AATACGACTCACTATAG
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-B-frSkyran-S was a gift from Shiro Suetsugu (Addgene plasmid # 182209 ; http://n2t.net/addgene:182209 ; RRID:Addgene_182209)

  • For your References section:

    Development of a green reversibly photoswitchable variant of Eos fluorescent protein with fixation resistance. Osuga M, Nishimura T, Suetsugu S. Mol Biol Cell. 2021 Nov 1;32(21):br7. doi: 10.1091/mbc.E21-01-0044. Epub 2021 Sep 8. 10.1091/mbc.E21-01-0044 PubMed 34495704
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