AeU6 3p3 GFP LgRNA
(Plasmid
#182208)
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PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAaU6-LgRNA
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xP3-GFP
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gRNA/shRNA sequenceN/A
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer U6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AeU6 3p3 GFP LgRNA was a gift from Craig Montell (Addgene plasmid # 182208 ; http://n2t.net/addgene:182208 ; RRID:Addgene_182208) -
For your References section:
A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti. Chen J, Luo J, Gurav AS, Chen Z, Wang Y, Montell C. Genetics. 2021 Nov 5;219(3). pii: 6368066. doi: 10.1093/genetics/iyab146. 10.1093/genetics/iyab146 PubMed 34740249
Map uploaded by the depositor.
