DmU6 3p3 GFP LgRNA
(Plasmid
#182207)
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PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDmU6-LgRNA
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3XP3-GFP
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gRNA/shRNA sequenceN/A
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DmU6 3p3 GFP LgRNA was a gift from Craig Montell (Addgene plasmid # 182207 ; http://n2t.net/addgene:182207 ; RRID:Addgene_182207) -
For your References section:
A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti. Chen J, Luo J, Gurav AS, Chen Z, Wang Y, Montell C. Genetics. 2021 Nov 5;219(3). pii: 6368066. doi: 10.1093/genetics/iyab146. 10.1093/genetics/iyab146 PubMed 34740249