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Anti-GluN1 [N308/48R]
(Antibody #182200)

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Western Blot image by James Trimmer, UC Davis

Image 1 (of 2): Western Blot
Crude rat membranes immunoblotted against Anti-GluN1 [N308/48R] hybridoma-derived antibod… View details
Credit: James Trimmer, UC Davis

Immunohistochemistry image by James Trimmer, UC Davis

Image 2 (of 2): Immunohistochemistry
Free-floating rat brain sections were fixed and stained with purified Anti-GluN1 [N308/48… View details
Credit: James Trimmer, UC Davis

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 182200-rAb 100 µg of purified recombinant antibody $250 *
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Recombinant Antibody trial size 182200-rAb.T 20 µg of purified recombinant antibody $85 *
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Features

Production & Usage

  • Production Plasmid
    Anti-GluN1 [N308/48R]
  • Antigen Plasmid
    pCI-SEP-NRI
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2909568

Terms and Licenses

Target Antigen

Protein Glutamate receptor ionotropic, NMDA 1
Antigen Description Amino acids 42–361 (extracellular N-terminus) of rat GluN1/NR1
Antigen Amino Acid Sequence
FREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKL
Species R. norvegicus (rat)
Additional Species Reactivity H. sapiens (human),  M. musculus (mouse)
Gene Grin1
Alternative Names
  • GluN1
  • Glutamate [NMDA] receptor subunit zeta-1
  • N-methyl-D-aspartate receptor subunit NR1
  • NMD-R1
External References

Applications (1)

Information
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Western Blot

Recommended
1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
H. sapiens (human)
Result
Pass
View Details
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-GluN1 [N308/48R] - from James Trimmer (Addgene antibody # 182200 ; http://n2t.net/addgene:182200 ; RRID:AB_2909568)

  • For your References section:

    High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930