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Addgene

p{Mhc-F4-678-EGFP::GW,attB}
(Plasmid #182170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Other

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.

Please note: The plasmid contains an AT nucleotide deletion in the AT-repeat region of Mhc-F4-678-EGFP::GW. This deletion is not expected to have any functional consequences on the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p{Mhc-F4-678-EGFP::GW,attB} was a gift from David Stern (Addgene plasmid # 182170 ; http://n2t.net/addgene:182170 ; RRID:Addgene_182170)

  • For your References section:

    Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species. Stern DL. G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 10.1093/g3journal/jkac030. 10.1093/g3journal/jkac030 PubMed 35143616
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