pTol2-eab2-GG
(Plasmid
#182154)
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PurposeExpression of artificial intron separated eGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTol2-eab2
- Backbone size w/o insert (bp) 6567
- Total vector size (bp) 7633
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGG
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Alt nameN-eGFP-intron-C-eGFP
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SpeciesSynthetic
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Insert Size (bp)1066
- Promoter eab2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer GCAGAGCAAAACAGGAAGTTGACTC
- 3′ sequencing primer GCATCACAAATTTCACAAATAAAGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2-eab2-GG was a gift from Hui Zong (Addgene plasmid # 182154 ; http://n2t.net/addgene:182154 ; RRID:Addgene_182154) -
For your References section:
zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing. Xu B, Kucenas S, Zong H. Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). pii: 2122529119. doi: 10.1073/pnas.2122529119. 10.1073/pnas.2122529119 PubMed 35197298