pMS_375
(Plasmid #182132)

• Purpose: expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR
• Depositing Lab: George Church
• Publication: Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118(18). pii: 2018181118. doi: 10.1073/pnas.2018181118.

Backbone

• Vector backbone: Sc101 cat araC pBAD
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 6802

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: retron RNA
• Species: escherichia coli BL21
• Insert Size (bp): 236
• Mutation: incorporated rifampicin-resistance-conferring donor

Gene/Insert 2

• Gene/Insert name: ec.86 RT
• Species: escherichia coli BL21
• Insert Size (bp): 963

Gene/Insert 3

• Gene/Insert name: CspRecT
• Species: collinsella stercoris prophage
• Insert Size (bp): 813

Resource Information

• Supplemental Documents:
  • pms_375 csprecT retron, with mutL_.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry