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Addgene

pMS_366
(Plasmid #182131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Sc101 cat araC pBAD
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 6802

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    retron RNA
  • Species
    escherichia coli BL21
  • Insert Size (bp)
    236
  • Mutation
    incorporated rifampicin-resistance-conferring donor

Gene/Insert 2

  • Gene/Insert name
    ec.86 RT
  • Species
    escherichia coli BL21
  • Insert Size (bp)
    963

Gene/Insert 3

  • Gene/Insert name
    CspRecT
  • Species
    collinsella stercoris prophage
  • Insert Size (bp)
    813

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS_366 was a gift from George Church (Addgene plasmid # 182131 ; http://n2t.net/addgene:182131 ; RRID:Addgene_182131)

  • For your References section:

    High-throughput functional variant screens via in vivo production of single-stranded DNA. Schubert MG, Goodman DB, Wannier TM, Kaur D, Farzadfard F, Lu TK, Shipman SL, Church GM. Proc Natl Acad Sci U S A. 2021 May 4;118(18). pii: 2018181118. doi: 10.1073/pnas.2018181118. 10.1073/pnas.2018181118 PubMed 33906944
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