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Addgene

3xNLS-NLP-cMyc-cMyc AspCas12a
(Plasmid #182122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5343
  • Total vector size (bp) 9495
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xNLS-NLP-cMyc-cMyc AspCas12a
  • Alt name
    3xNLS AspCas12a
  • Species
    Acidaminococcus
  • Insert Size (bp)
    4152
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xNLS-NLP-cMyc-cMyc AspCas12a was a gift from Scot Wolfe (Addgene plasmid # 182122 ; http://n2t.net/addgene:182122 ; RRID:Addgene_182122)

  • For your References section:

    Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells. Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA. GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 10.1089/genbio.2022.0003. Epub 2022 Jun 14. 10.1089/genbio.2022.0003 PubMed 38405215
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