IRSp53/BAIAP2 scFv [L117/1]
(Plasmid
#182075)
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PurposeMammalian Expression of IRSp53/BAIAP2 scFv. Derived from hybridoma L117/1.
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerecombinant mouse scFv targeting IRSp53/BAIAP2 (Homo sapiens)
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SpeciesM. musculus (mouse)
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Entrez GeneBAIAP2 (a.k.a. BAP2, FLAF3, IRSP53, WAML)
- Promoter CMV
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Tag
/ Fusion Protein
- Sortase, His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a recombinant scFv derived from RRID: AB_2895682.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IRSp53/BAIAP2 scFv [L117/1] was a gift from James Trimmer (Addgene plasmid # 182075 ; http://n2t.net/addgene:182075 ; RRID:Addgene_182075) -
For your References section:
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930