pcDNA3.1(+)-mTagBFP2-MRTF-B
(Plasmid #182062)

• Purpose: Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.
• Depositing Lab: Jin Zhang
• Publication: Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. doi: 10.1371/journal.pbio.3000866. eCollection 2020 Sep.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5355
• Total vector size (bp): 9318
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mTagBFP2-MRTF-B
• Species: M. musculus (mouse)
• Insert Size (bp): 3963
• Entrez Gene: Mrtfb (a.k.a. Gt4-1, MRTF-B, Mkl2, SOLOIST, mKIAA1243)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTagBFP2 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+)-TagBFP-MRTF-B.gb
• A portion of this plasmid was derived from a plasmid made by: Mrtbf cDNA was provided by T.W.J. Gadella (University of Amsterdam)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry