pAAV-pgk-DIO-rvCRY-WPRE
(Plasmid
#182060)
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PurposePlasmids for production of AAV-based TFactivity reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
- Promoter hu Pgk1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-pgk-DIO-rvCRY-WPRE was a gift from Kentaro Abe (Addgene plasmid # 182060 ; http://n2t.net/addgene:182060 ; RRID:Addgene_182060) -
For your References section:
PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery. Abe H, Abe K. iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927. eCollection 2022 Mar 18. 10.1016/j.isci.2022.103927 PubMed 35281741