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pCDH1-CMV-HT-LC3-SV40-Hygro
(Plasmid #182045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH1-CMV-MCS-SV40-Hygro
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP1LC3B
  • Alt name
    LC3B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    378
  • GenBank ID
    NM_022818.5
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GAA​GAT​TCT​AGA​ACC​ATG​GCA​GAA​ATC​GG
  • 3′ sequencing primer TAT​AGG​ATC​CCT​ACA​CTG​ACA​ATT​TCA​TCC​CG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH1-CMV-HT-LC3-SV40-Hygro was a gift from Hong-Gang Wang (Addgene plasmid # 182045 ; http://n2t.net/addgene:182045 ; RRID:Addgene_182045)

  • For your References section:

    VPS37A directs ESCRT recruitment for phagophore closure. Takahashi Y, Liang X, Hattori T, Tang Z, He H, Chen H, Liu X, Abraham T, Imamura-Kawasawa Y, Buchkovich NJ, Young MM, Wang HG. J Cell Biol. 2019 Oct 7;218(10):3336-3354. doi: 10.1083/jcb.201902170. Epub 2019 Sep 13. 10.1083/jcb.201902170 PubMed 31519728
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