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MBP_Lims (MBP_LIM1234)
(Plasmid #182042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MBP vektor
  • Total vector size (bp) 7335
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    RT or 18C O/N (150 rpm). BL21-Star (DE3) is recommended for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LIM1234
  • Alt name
    Paxillin
  • Alt name
    PXN
  • Species
    H. sapiens (human)
  • Mutation
    LIM domains 1-4 only
  • Entrez Gene
    PXN
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

collaboration project

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP_Lims (MBP_LIM1234) was a gift from Vesa Hytönen (Addgene plasmid # 182042 ; http://n2t.net/addgene:182042 ; RRID:Addgene_182042)

  • For your References section:

    Structural and functional analysis of LIM domain-dependent recruitment of paxillin to alphavbeta3 integrin-positive focal adhesions. Ripamonti M, Liaudet N, Azizi L, Bouvard D, Hytonen VP, Wehrle-Haller B. Commun Biol. 2021 Mar 29;4(1):380. doi: 10.1038/s42003-021-01886-9. 10.1038/s42003-021-01886-9 PubMed 33782527
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