GST_LIM1224
(Plasmid #182041)

• Purpose: For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.
• Depositing Lab: Vesa Hytönen
• Publication: Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi: 10.1038/s42003-021-01886-9.

Backbone

• Vector backbone: pGST-2T
• Total vector size (bp): 5654
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: RT or 18C O/N (150 rpm)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LIM1224
• Alt name: Paxillin
• Alt name: PXN
• Species: H. sapiens (human)
• Mutation: LIM domains 1, 2, 2 and 4
• Entrez Gene: PXN
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 1) 2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

collaboration project