pRIV-Gal4DBD:2xnMagHigh1
(Plasmid
#182031)
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PurposeDonor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRIV vector
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Vector typeInsect Expression ; donor plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal4DBD2xHighMag1-p103'UTR
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Insert Size (bp)2126
Resource Information
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A portion of this plasmid was derived from a plasmid made byMoritoshi Sato lab (fungal photoreceptor gene from https://pubmed.ncbi.nlm.nih.gov/25708714/).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRIV-Gal4DBD:2xnMagHigh1 was a gift from Jean-Paul Vincent (Addgene plasmid # 182031 ; http://n2t.net/addgene:182031 ; RRID:Addgene_182031) -
For your References section:
Rapid and robust optogenetic control of gene expression in Drosophila. di Pietro F, Herszterg S, Huang A, Bosveld F, Alexandre C, Sancere L, Pelletier S, Joudat A, Kapoor V, Vincent JP, Bellaiche Y. Dev Cell. 2021 Dec 20;56(24):3393-3404.e7. doi: 10.1016/j.devcel.2021.11.016. Epub 2021 Dec 7. 10.1016/j.devcel.2021.11.016 PubMed 34879263