pAAV-CAG-HcKCR2-EYFP
(Plasmid
#182022)
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PurposeExpress light-gated potassium channel HcKCR2-EYFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-CAG(short)-WPRE-hGH
- Backbone size w/o insert (bp) 5940
- Total vector size (bp) 6735
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHcKCR2
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SpeciesSynthetic
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Insert Size (bp)795
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-HcKCR2-EYFP was a gift from Mingshan Xue (Addgene plasmid # 182022 ; http://n2t.net/addgene:182022 ; RRID:Addgene_182022) -
For your References section:
Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition. Govorunova EG, Gou Y, Sineshchekov OA, Li H, Lu X, Wang Y, Brown LS, St-Pierre F, Xue M, Spudich JL. Nat Neurosci. 2022 Jun 20. pii: 10.1038/s41593-022-01094-6. doi: 10.1038/s41593-022-01094-6. 10.1038/s41593-022-01094-6 PubMed 35726059