T4 Lysozyme I3C I9C T21C C54T T142C L164C
(Plasmid
#18202)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 18202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHS1403
- Backbone size w/o insert (bp) 5000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameT4 Lysozyme
-
Alt nameD3921
-
SpeciesBacteriophage T4
-
Insert Size (bp)600
-
MutationI3C I9C T21C C54T T142C L164C
-
GenBank IDNP_049736
-
Entrez Genee (a.k.a. T4p123)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer GGCGTATCACGAGGCCC (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Addgene sequencing results show that the plasmid lacks one of the two expected Tac promoters.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
T4 Lysozyme I3C I9C T21C C54T T142C L164C was a gift from Brian Matthews (Addgene plasmid # 18202 ; http://n2t.net/addgene:18202 ; RRID:Addgene_18202)