pcDNA5/FRT/TO_STf_SKL
(Plasmid
#182007)
-
PurposeCMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 182007 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA5/FRT/TO
- Backbone size w/o insert (bp) 5100
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSTf_SKL
-
SpeciesSynthetic
-
Insert Size (bp)600
-
MutationFast mutation on SNAP-tag E30R
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5/FRT/TO_STf_SKL was a gift from Kai Johnsson (Addgene plasmid # 182007 ; http://n2t.net/addgene:182007 ; RRID:Addgene_182007) -
For your References section:
Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes. Frei MS, Koch B, Hiblot J, Johnsson K. ACS Chem Biol. 2022 Jun 17;17(6):1321-1327. doi: 10.1021/acschembio.2c00041. Epub 2022 May 18. 10.1021/acschembio.2c00041 PubMed 35584304